Cirriculum Vitae

Jonathan Frederick Clinthorne, B.S.

Department of Food Science & Human Nutrition

229 G.M. Trout Building, Michigan State University, East Lansing, MI 48824

Cell Phone: (734) 255-6295                                        Email: clinthor@msu.edu

Education:

Michigan State University                            East Lansing, MI                               August 2013 (expected)

Doctor of Philosophy in Human Nutrition.  Dissertation Title:  Dietary manipulation of natural killer cell biology through refeeding calorie restricted mice. 

Michigan State University                            East Lansing, MI                               May 2008

            Bachelors of Science degree with University Honors in Human Biology

Research Experience                            

May 2009 – Present                                       Michigan State University                East Lansing, MI

Predoctoral fellow to Dr. Elizabeth Gardner

• My research has focused on the role of energy intake and adipokines on immune homeostasis, with a specific emphasis on natural killer cell development and function in calorie restricted mice.  This research has been expanded to investigate the role of energy intake on immunity to influenza virus in a mouse model.
• Developed flow cytometric based methods for analyzing protein phosphorylation, and leukocyte proliferation, apoptosis, trafficking, cytotoxicity, and cytokine production.   
• Extensive familiarity using BD Biosciences FACS Canto II, LSR II, and Accuri C6 flow cytometers.  Analyzed 10 color flow cyotmetry data using FlowJo software.
• Utilized ELISA, magnetic cell sorting, RNA isolation from blood and tissues, methods for viral quantification (RT-PCR, MDCK assay), and Echo Magnetic Resonance Imaging for murine body composition analysis
• Collaborated on multiple project involving diet and immune function, with emphasis on the role of dietary interventions in reducing the severity of colitis and colorectal cancer in SMAD3-/- mice
• Committee members:  Dr. Elizabeth Gardner (PI), Dr. Jenifer Fenton, Dr. John Gerlach, Dr. Norman Hord, Dr. Peter Mancuso

May 2008-April 2009                                    Michigan State University                East Lansing, MI

Nutritional Immunology Lab Research Technician

• Participated in writing and submitting a successfully funded RO1 grant to the NIA 
• Assisted graduate student in studies involving the immunity of aged mice to influenza infection.
• Responsible for implementing dietary paradigms for study of caloric restriction in mice.

           

Teaching experience:

January 2013 – Present                                Michigan State University                East Lansing, MI

Teaching Assistant    

• Food Microbiology Laboratory – Designed and instructed students on methods for studying major groups of microorganisms important to the food industry with emphasis on food safety.  Isolation, enumeration, characterization, identification, and use of microorganisms in a laboratory setting using classical and molecular microbiology techniques (Seniors)

Jan 2012 & 2013                                            Michigan State University                East Lansing, MI

Guest Lecturer

• Medical Nutritional Therapy - Two fifty-minute lectures to dietetics students:  “Introduction to Acid/Base Physiology” (Seniors and graduate students)

                                         

Feb 2011                                                         Michigan State University                East Lansing, MI

Guest Lecturer

• One ninety-minute lecture to nutritional sciences students: “The Role of Leptin in Natural Killer Cell Homeostasis and Function”  (Seniors)

Professional Experience

August 2012 – Present                                  Michigan State University                East Lansing, MI

Graduate Student Organizer

• Organize events for graduate students to interact in a social manner.  Welcome new graduate students to the department.  Lead discussions with invited speakers at departmental lunches.

March 2011 – Present                                   Journal of Physiology and Behavior

Reviewer

• Invited reviewer 

Jan 2011 – December 2011                           American Society for Nutrition

Student Blogger

• Wrote blogs published on the American Society of Nutrition website.  Focused on the role of nutrition in the prevention of obesity and exercise physiology.

April 2010-April 2011                                   American Society for Nutrition

Student Representative

• Student coordinator for Nutritional Immunology Research Interest Section.  Graded and programmed abstracts, assisted Research Interest Section chair and secretary on organizing meetings and minisymposium.

October 2007 - May 2008                             YMCA                                               Lansing, MI

Nutrition and Fitness Coach and Mentor

• Educated and counseled at risk youth in healthy lifestyle choices.  Designed and lead fitness routines and provided access to nutritional information. 

Professional Society Memberships:

2011-Present              International Behavioral Neuroscience Society

2009-Present              American Society for Nutrition (ASN)

-          Ad hoc reviewer, Journal of Nutrition

Honors/Awards:

• Atrium Innovations Trainee Award, 2012
• American Society for Nutrition, Nutritional Immunology Trainee Travel Award, 2011
• Department of Food Science and Human Nutrition, John Harvey Kellogg Fellow, 2011, 2012
• Department of Food Science and Human Nutrition, Gozenbach Fellowship for Graduate Mentoring and Research, 2010
• College of Natural Sciences, Early Start Dissertation Fellowship, 2009
• Graduated with Honors, Michigan State University, 2008

Peer Reviewed Publications:

Clinthorne JF, Gardner EM.  Rapamycin and caloric restriction inhibit terminal NK cell maturation in vitro and in vivo.  In preparation.

Clinthorne JF, Duriancik DM, Gardner EM.  Caloric restriction suppresses B lymphopoiesis and enhances granulopoiesis in murine bone marrow.  Submitted to J Leukocyte Biology..

Clinthorne JF, Beli E, Duriancik DM, Gardner EM. NK cell maturation and function in C57BL/6 mice are altered by caloric restriction. J Immunol. 2013 Jan 15;190(2):712-22.

Gurzell EA, Teague H, Harris M, Clinthorne JF, Shaikh SR, Fenton JI. DHA-enriched fish oil targets B cell lipid microdomains and enhances ex vivo and in vivo B cell function. J Leukoc Biol.  2012 Nov 24.

Gopalakrishnan A, Clinthorne JF, Rondini EA, McCaskey SJ, Gurzell EA, Langohr IM, Gardner EM, Fenton JI. Supplementation with galacto-oligosaccharides increases the percentage of NK cells and reduces colitis severity in Smad3-deficient mice. J Nutr. 2012 Jul;142(7):1336-42.

McCaskey SJ, Rondini EA, Clinthorne JF, Langohr IM, Gardner EM, Fenton JI. Increased presence of effector lymphocytes during Helicobacter hepaticus-induced colitis. World J Gastroenterol.  2012 Apr 7;18:1459-69.

Beli E, Clinthorne JF, Duriancik DM, Hwang I, Kim S, Gardner EM.  Natural killer cell function is altered during the primary response of aged mice to influenza.  Mech Ageing Dev. 2011 Oct;132(10):503-10.

Gardner EM, Beli E, Clinthorne JF, Duriancik DM. 2011. Energy intake and response to infection with influenza. Ann Rev Nutr. 2011 Aug 21;31:353-67.

Woodworth HL, McCaskey SJ, Duriancik DM, Clinthorne JF, Langohr IM, Gardner EM, Fenton JI. Dietary fish oil alters T lymphocyte cell populations and exacerbates disease in a mouse model of inflammatory colitis. Cancer Res.  2010 Oct 15;70:7960-9

Clinthorne, JF, Adams DJ, Fenton JI, Ritz BW, Gardner EM. "Short-Term Re-Feeding of Previously Energy-Restricted C57BL/6 Male Mice Restores Body Weight and Body Fat and Attenuates the Decline in Natural Killer Cell Function after Primary Influenza Infection." J Nutr. 2010 Aug;140(8):1495-501

Presentations:

(Published Presentation Abstracts) * indicates presenter

Clinthorne JF *, Gardner, EM.  2012.  Energy Restriction Results in Phenotypic and Functional Alterations to NK Cells.  Federation of American Societies for Experimental Biology Meeting, San Diego, CA.  (Talk)

Beli E*, Clinthorne, JF, Duriancik, DM; et al.  2012.  A comparison of young and aged NK cells at basal, homeostatic conditions in C57BL/6 mice.  Federation of American Societies for Experimental Biology Meeting, San Diego, CA.  (Poster)

Clinthorne JF *, Gardner EM. 2011. Refeeding of previously young energy restricted mice reveals relationship between NK cell homeostasis and energy intake. Federation of American Societies for Experimental Biology Meeting. Washington, DC.  (Poster)

Clinthorne JF*, Beli E, Duriancik DM, and Gardner EM. 2010. Refeeding an ad libitum diet to energy restricted C57BL/6 mice restores natural killer cell numbers and percentages during primary influenza infection. Federation of American Societies for Experimental Biology Meeting, Anaheim, CA.   (Talk)

Duriancik DM*, Clinthorne JF, Gopalakrishnan A, Fenton JI, and Gardner EM. 2010. Galacto-Oligosaccarhides modulate gastrointestinal and systemic lymphocyte populations of SMAD3 knockout mice. Federation of American Societies for Experimental Biology Meeting. Anaheim, CA.  (Talk)

Woodworth HL*, McCaskey SJ, Duriancik DM, Clinthorne JF, Langohr IM, Gardner EM, Fenton JI. 2010. DHA Exacerbates Experimentally Induced Colitis in SMAD3-/- Mice. Federation of American Societies for Experimental Biology Meeting, Anaheim, CA.  (Poster)

Invited Presentations:

Trail Summit 2013.  “Dietary Recommendations to Limit GI Distress During Trail Running – Low FODMAP diets”, Three Rivers Running Company, Fort Wayne, IN, scheduled March 2013.  (Talk)

Trail and Ultrarunning Summit 2012.  “Evidence Based Sports Nutrition Recommendations”, Fort Wayne, IN, May 2012.  (Talk)

Marathon 501.  “Nutrition Advice for Before, During, and After the Long Run”,  Runningfit, Bloomington, MI, February 2012.  (Talk)